Use of enzymes obtained through the sustainable cultivation of mold on solid organic waste for the utilization of underexploited agricultural materials under a zero-waste approach

Authors

  • Manuel Carrillo Cárdenas Universidad Politécnica de Huejutla
  • Jazmín Hernández Hernández Universidad Autónoma Chapingo
  • Anita Hernández Hernández Universidad Politécnica de Huejutla
  • María Isabel Hernández Hernández Universidad Politécnica de Huejutla

DOI:

https://doi.org/10.59057/iberoleon.20075316.202440755

Keywords:

enzymes, sustainable cultivation, organic waste, agricultural utilization, zero waste

Abstract

The accelerated exploitation of natural recourses has led the humanity towards a con­cerning situation, as some resources will be completely depleted soon. This will carry undesirable consequences not only for our species, but also for the whole planet. There­fore, the development of procedures that allow the recovery of useful substances from unexploited sources such as wastes has become an urgent issue. This paper presents a simple, low-cost, sustainable, and easily replicable method to produce cellulases and pectinases from mold cultures by means of solid-state fermentation. These enzymes can be used for the recovery of valuable compounds from agriculture wastes. Particu­larly, the recovery of carotenoids from mango peel and the production of fermentable sugars from chote fruit cellulose fibers are presented. By doing so, a zero-waste strategy is adopted, where every waste is seen as a potential raw material. The role of three young women, who are currently agroindustrial engineers, in the development of the project is highlighted, as this contributes to the gender parity in science.

Downloads

Download data is not yet available.

References

Boondaeng, A., Keabpimai, J., Trakunjae, C., Vaithanomsat. P. y Srichola, P., Niyomvong, N. (2024). Cellulase production under solid-state fermentation by Aspergillus sp. IN5: Parameter optimization and application. Heliyon, 10, e26601. https://doi.org/10.1016/j.heliyon.2024.e26601.

Buckley, M. (2008). The fungal kingdom: Diverse and essential roles in earth´ s ecosystem. American Socetiy for Microbiology. https://doi.org/10.1128/aamcol.2nov.2007.

Castillo-Ruíz, R., Castillo-Archilla, J. A., Alemán-Castillo, S. E., Castillo-Ruiz, O. y Trejo-Díaz, G. N. (2022). Alternativas para el aprovechamiento del cuajilote (Parmentiera edulis): Una especie agroindustrial subutilizada. CienciaUAT. https://doi.org/10.29059/cienciauat.v17i1.1635.

Chilakamarry, C. R., Mimi Sakinah, A. M., Zularisam, A. W., Sirohi, R., Khilji, I. A., Ahmad, N. y Pandey, A. (2022). Advances in solid-state fermentation for bioconversion of agricultural wastes to value-added products: Opportunties and challenges. Bioresource Technology, 343, 126065. https://doi.org/10.1016/j.biortech.2021.126065.

Datta, S., Christena, L. R. y Rajaram, Y. R. S. (2013). Enzyme immobilization: an overview on techniques and support materials. 3 Biotech, 3(1), 1-9. https://doi.org/10.1007/s13205-012-0071-7.

De Souza, T. S. P. y Kawaguti, H. Y. (2021). Cellulases, hemicellulases, and pectinases: applications in the food and beverage industry. Food Bioprocess Technology, 14, 1446-1477. https://doi.org/10.1007/s11947-021-02678-z.

El-Gendi, H., Saleh, A. K., Badierah, R., Redwan, E. M., El-Maradny, Y. A. y El-Fakharany, E. M. (2022). A comprehensive insight into fungal enzymes: structure, classification, and their role in mankind’s challenges. Journal of Fungi, 8, 23. https://doi.org/10.3390/jof8010023.

Evviva Sciences. (2019). Mold identification guide. Fiedor, J., y Burda, K. (2014). Potential role of carotenoids as antioxidants in human health and disease. Nutrients, 6(2), 466-488. https://doi.org/10.3390/nu6020466.

Gebregziabher, B. S., Gebremeskel, H., Debesa, B., Ayalneh, D., Mitiku, T., Wendwessen, T., Habtemariam, E., Nur, S. y Getachew, T. (2023). Carotenoids: Dietary sources, health functions, biofortification, marketing trend and affecting factors – A review. Journal of Agriculture and Food Research, 14, 2666-1543. https://doi.org/10.1016/j.jafr.2023.100834.

Hernández-López, A., Sanchez Felix, D. A., Sierra, Z. Z., Bravo, I. G., Dinkova, T. D. y Avila-Alejandre, A. X. (2020). Quantification of reducing sugars based on the qualitative technique of Benedict. ACS Omega, 5(50), 32403-32410. https://doi.org/10.1021/acsomega.0c04467.

Katoch, M., Singh, G. y Vishwakarma R. A. (2014). Molecular Mechanism of Cellulase Production Systems in Trichoderma. En V. K. Gupta, M. Schmoll, A. Herrera-Estrella, R. S. Upadhyay, I. Druzhinina y M. G. Tuohy (Eds.), Biotechnology and Biology of Trichoderma (pp. 319-324). Elsevier. https://doi.org/10.1016/B978-0-444-59576-8.00022-9.

Kopec, R. E., Cooperstone, J. L., Cichon, M. J. y Schwartz, S. J. (2012). Analysis methods of carotenoids. En Z. Xu y L. R. Howard (Ed.), Analysis of Antioxidant Rich Phytochemicals (pp. 105-148). John Wiley & Sons.

Marín, M., Sánchez, A. y Artola, A. (2019). Production and recovery of cellulases through solid-state fermentation of selected lignocellulosic wastes. Journal of Cleaner Production, 209, 937-946. https://doi.org/10.1016/j.jclepro.2018.10.264.

Meléndez-Martínez, A. J., Mandić, A. I., Bantis, F., Böhm, V., Borge, G. I. A., Brnčić, M., … O’Brien, N. (2021). A comprehensive review on carotenoids in foods and feeds: Status quo, applications, patents, and research needs. Critical Reviews in Food Science and Nutrition, 62(8), 1999-2049. https://doi.org/10.1080/10408398.2020.1867959.

Mrudula, S. y Murugammal, R. (2011). Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate. Brazilian Journal of Microbiology, 42(3), 1119-1127.

Palacios-Hinestroza, H., del Real-Olvera, J., Gurubel-Tun, K. J. y Sulbarán-Rangel, B. (2020). Biocombustibles a partir de la biomasa lignocelulósica. En F. Navarrete-Báez (Coord.), La eficiencia de las energías renovables en México (pp. 51-59). Universidad del Valle de Atemajac.

Pandey, A. K. y Negi, S. (2020). Enhanced cellulase recovery in SSF from Rhizopus oryzae SN5 and immobilization for multi-batch saccharification of carboxymethylcellulose. Biocatalysis and Agricultural Biotechnology, 26, 101656. https://doi.org/10.1016/j.bcab.2020.101656.

Pérez-Morales, S. (2020). Caracterización química y nutricional del fruto de chote (Parmentiera aculeata) [Tesis de maestría, Colegio de Postgraduados]. Repositorio institucional. http://colposdigital.colpos.mx:8080/xmlui/bitstream/handle/10521/4976/Perez_Morales_S_MC_RGP_Ganaderia_2020.pdf?sequence=1&isAllowed=y.

Pietzsch, N., Duarte Ribeiro, J. L. y de Medeiros, J. F. (2017). Benefits, challenges and critical factors of success of Zero Waste: A systematic literature review. Waste Management, 67, 324-353. https://doi.org/10.1016/j.wasman.2017.05.004.

Rivera-Madrid, R., Carballo-Uicab, V. M., Cárdenas-Conejo, Y., Aguilar-Espinosa, M. y Ramamoorthy Siva, R. (2020). 1 – Overview of carotenoids and beneficial effects on human health. En C. M. Galanakis (Ed.), Carotenoids: Properties, Processing and Applications (pp. 1-40). Academic Press. https://doi.org/10.1016/B978-0-12-817067-0.00001-4.

Sánchez-Camargo, A. P., Gutiérrez, L. F., Vargas, S. M., Martínez-Correa, H. A., Parada-Alfonso, F. y Narváez-Cuenca, C. E. (2019). Valorization of mango peel: Proximate composition, supercritical fluid extraction of carotenoids, and application as an antioxidant additive for an edible oil. The Journal of Supercritical Fluids, 152, 104574. https://doi.org/10.1016/j.supflu.2019.104574.

Santiago Ruiz, C., Nuricumbo Lievano, V. N., Chapa Barrios, M. G., Vela Gutiérrez, G. y Velázquez, A. (2021). Antimicrobial activity, phenolic and antioxidant content of extracts from cuajilote (Parmentiera aculeata Kunth) fruits at different degrees of ripening. Journal of the Mexican Chemical Society, 65(2), 161-169. http://dx.doi.org/10.29356/jmcs.v65i2.1270.

Segura Salazar, C. M., Soto Espinoza, Y., Castillejos López, W. y Badillo Guzmán, J. (2022), Mujeres indígenas: Experiencias y sentidos en torno a la universidad. Diálogos sobre Educación, 13(25), 1-17. https://doi.org/10.32870/dse.v0i25.1134.

Singh, R., Das, R., Sangwan, S., Rohatgi, B., Khanam, R., Kulsum P. S., Das, S., Lyngdoh, Y.A., Langyan, S., Shukla, A., Shrivastava, M. y Misra, S. (2021). Utilisation of agro-industrial waste for sustainable green production: A review. Environmental Sustainability, 4, 619-636. https://doi.org/10.1007/s42398-021-00200-x.

Singh, B., Soni, S. K., Mathur, P. y Garg, N. (2024). Microbial multienzyme viz., pectinase, cellulase and amylase production using fruit and vegetable waste as substrate. A review. Applied Microbiology, 4(3), 1232-1246. https://doi.org/10.3390/applmicrobiol4030084.

Vilas-Franquesa, A., Fryganas, C., Casertano, M., Nontemurro, M. y Fogliano, V. (2024). Upcycling mango peels into a functional ingredient by combining fermentation and enzymatic-assisted extraction. Food Chemistry, 434, 137515. https://doi.org/10.1016/j.foodchem.2023.137515.

Wang, K., Shen, Z., Wang, X., Li, Z. y Cheng, S. (2024). Advances in enhancing the enzymatic saccharification process of lignocellulosic biomasses for bioethanol production. Biomass and Bioenergy, 191, 107450. https://doi.org/10.1016/j.biombioe.2024.107450.

Yadav, M. y Ahmadi, Y. (2019). Alginates: Source, chemistry and properties. En M. S. Hasnain y A. K. Nayak (Ed.), Alginates. Versatile polymers in biomedical applications and therapeutics. Apple Academic Press.

Yara-Varón, E., Li, Y., Balcells, M., Canela-Garayona, R., Fabiano-Tixier, A-S. y Chemat, F. (2017). Vegetable oils as alternative solvents for green oleo-extraction, purification and formulation of food and natural products. Molecules, 22(9), 1474. https://doi.org/10.3390/molecules22091474.

Published

2024-12-18

How to Cite

Carrillo Cárdenas, M., Hernández Hernández, J., Hernández Hernández, A., & Hernández Hernández, M. I. (2024). Use of enzymes obtained through the sustainable cultivation of mold on solid organic waste for the utilization of underexploited agricultural materials under a zero-waste approach. Entretextos, 16(40), 1–19. https://doi.org/10.59057/iberoleon.20075316.202440755